polyclonal rabbit anti phosphorylated s6 ribosomal protein Search Results


rabbit anti-human phosphorylated ribosomal protein s6 (ser235/236) gtx113542  (GeneTex)
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GeneTex rabbit anti-human phosphorylated ribosomal protein s6 (ser235/236) gtx113542
(A). The skin cell morphology in the anal and tail fin regions of WT (a-d) or cdc6/c-myc double transgenics (e-h) aged 8 dpf. To facilitate skin cell counting, WT, cdc6- and c-myc-overexpressing fish were crossed with Tg( krt4 :nlsEGFP) cy34 and their skin cell density were compared. The positions labeled by dotted line in the anal (f) and tail (g) fin of cdc6/c-myc transgenics were enlarged to show abnormal skin aggregation in cdc6/c-myc transgenics. The corresponding positions in WT were also shown (b and c). Total cell nuclei including skin cells were labeled by CENPF antibody (magenta color) (d and h) and skin cells were double labeled by CENPF and EGFP antibody (green color). Overexpression of cdc6/c-myc in skin cell can induce skin cell abnormal aggregation in dorsal and anal fin (stars in f, g, and h). (B) Comparison of the expression of cell cycle markers by real time RT-PCR. The cell cycle markers like ccnd1, ccne, and cdk2 were significantly upregulated while the cell cycle inhibitor cdkn1a was significantly downregulated. (C) Comparison of the protein expression of protein synthesis markers. The marker like <t>p-RPS6</t> was greatly upregulated in cdc6/c-myc transgenics. Result is presented as a representative of at least three independent experiments.
Rabbit Anti Human Phosphorylated Ribosomal Protein S6 (Ser235/236) Gtx113542, supplied by GeneTex, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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(A). The skin cell morphology in the anal and tail fin regions of WT (a-d) or cdc6/c-myc double transgenics (e-h) aged 8 dpf. To facilitate skin cell counting, WT, cdc6- and c-myc-overexpressing fish were crossed with Tg( krt4 :nlsEGFP) cy34 and their skin cell density were compared. The positions labeled by dotted line in the anal (f) and tail (g) fin of cdc6/c-myc transgenics were enlarged to show abnormal skin aggregation in cdc6/c-myc transgenics. The corresponding positions in WT were also shown (b and c). Total cell nuclei including skin cells were labeled by CENPF antibody (magenta color) (d and h) and skin cells were double labeled by CENPF and EGFP antibody (green color). Overexpression of cdc6/c-myc in skin cell can induce skin cell abnormal aggregation in dorsal and anal fin (stars in f, g, and h). (B) Comparison of the expression of cell cycle markers by real time RT-PCR. The cell cycle markers like ccnd1, ccne, and cdk2 were significantly upregulated while the cell cycle inhibitor cdkn1a was significantly downregulated. (C) Comparison of the protein expression of protein synthesis markers. The marker like p-RPS6 was greatly upregulated in cdc6/c-myc transgenics. Result is presented as a representative of at least three independent experiments.

Journal: Oncotarget

Article Title: Cdc6 cooperates with c-Myc to promote genome instability and epithelial to mesenchymal transition (EMT) in zebrafish

doi:

Figure Lengend Snippet: (A). The skin cell morphology in the anal and tail fin regions of WT (a-d) or cdc6/c-myc double transgenics (e-h) aged 8 dpf. To facilitate skin cell counting, WT, cdc6- and c-myc-overexpressing fish were crossed with Tg( krt4 :nlsEGFP) cy34 and their skin cell density were compared. The positions labeled by dotted line in the anal (f) and tail (g) fin of cdc6/c-myc transgenics were enlarged to show abnormal skin aggregation in cdc6/c-myc transgenics. The corresponding positions in WT were also shown (b and c). Total cell nuclei including skin cells were labeled by CENPF antibody (magenta color) (d and h) and skin cells were double labeled by CENPF and EGFP antibody (green color). Overexpression of cdc6/c-myc in skin cell can induce skin cell abnormal aggregation in dorsal and anal fin (stars in f, g, and h). (B) Comparison of the expression of cell cycle markers by real time RT-PCR. The cell cycle markers like ccnd1, ccne, and cdk2 were significantly upregulated while the cell cycle inhibitor cdkn1a was significantly downregulated. (C) Comparison of the protein expression of protein synthesis markers. The marker like p-RPS6 was greatly upregulated in cdc6/c-myc transgenics. Result is presented as a representative of at least three independent experiments.

Article Snippet: After blocking with 3% BSA/PBST at room temperature for 60 min, embryos were incubated at 4°C overnight with 1:200 diluted primary antibodies as follows: rabbit anti-pH3 (sc-8656-R, Santa Cruz), rabbit anti-human phosphorylated ribosomal Protein S6 (Ser235/236) (GTX113542, GeneTex), rabbit anti-human CK8 (GTX110311, Genetex), rabbit anti-human CK13 (GTX109883, Genetex), rabbit anti-human MMP9 (GTX100626, GeneTex), and rabbit anti-human CENPF (GTX100212, Genetex).

Techniques: Cell Counting, Labeling, Over Expression, Comparison, Expressing, Quantitative RT-PCR, Marker